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Exportin 4 depletion leads to nuclear accumulation of a subset of ... Fragmented DNA was purified and quantified by Qubit 3.0 (Invitrogen). Mix 1.8 µg DNA with 0.2 µg E. coli DNA (for normalization) in 500 μl 1 × TE. For RNase R treated group, 10 U RNase R ... Comparative Photoaffinity Profiling of Omega-3 Signaling Lipid Probes ... The fish oil constituent docosahexaenoic acid (DHA, 22:6 n-3) is a signaling lipid with anti-inflammatory properties. The molecular mechanisms underlying the biological effect of DHA are poorly understood. Here, we report the design, synthesis, and application of a complementary pair of bio-orthogonal, photoreactive probes based on the polyunsaturated scaffold DHA and its oxidative metabolite ... Genetic Engineering - Genome.gov Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a re...